Gear I have (or don't have and say, would like to have):
Lenses, filters and adapters: Lens/filter/adapter Filter size | Lens/filter/adapter Filter size Canon 100mm f/2.8L 67f | Canon EF-M 22mm f/2 43f Canon 180mm f/3.5L 72f | Canon EF-M 18-55mm 52f Canon 50mm f/1.4 58f | Canon EF-S 10-22mm 77f Canon 24-105L 77f | CP & ND 77m Raynox 250 (probably have) see-> | Raynox 150 (seems recommended a lot) Raynox: 43m rear 49f front The snap-on fits 52-67mm Nikon CFI Plan Achromat 10x NA 0.25 WD 10.5mm with M25x0.75 x 52mm [1] 52m Mitutoyo (Edmund) 10x NA 0.28 WD 33.5mm with M26x36tpi x 52mm [2] 52m Extenders: Canon EF 12 II Extension Tube Canon EF 25 II Extension Tube Canon EF 1.4x Extender Rings: 52m - 43f 52(h) $34 - ordered 3/9/2014 (67m, 72m) - 52f 67(h), 72(h) (43m, 52m) - 58f 43(b), 52(b) (58m, 72m, 77m) - 67f 58(b), 72(cheap), 77(cheap) (52m, 58m, 62m, 67m, 72m) - 77f 52(cheap), 58(h), 62(b), 67(b), 72(b) 67m - 67m (macro coupler) 67(cheap) 52m-M25x0.75 (this is the adapter for [1]) 52m-M26x36tpi (this is the adapter for [2]) EF - 58m (58mm Macro Reverse Ring Camera Mount Adapter) EF - 77m (77mm Macro Reverse Ring Camera Mount Adapter) Configurations: EF-77m => 10-22mm (reversed) EF-58m => 50mm (reversed) 100mm => 67m-67m => 67f-43m => 22mm ----------------- The 67-43 was 6-8 wk delayed 100mm => 67m-67m => 67f-58m => 58f-43m => 22mm -- So, do instead 100mm => 67m-67m => 67f-58m => 50mm 100mm => 67m-67m => 67f-77m => 10-22mm 100mm => 67m-52f => 52m-M25x0.75 => Nikon 10x 100mm => 67m-52f => 52m-M26x36tpi => Mitutoyo 10x 100mm => 67m-52f => 52m-43f => Raynox 250 180mm => 72m-67f => 67m-67m => 67f-43m => 22mm ------ The 67-43 was 6-8 wk delayed 180mm => 72m-67f => 67m-67m => 67f-58m => 58f-43m => 22mm -- So, do instead 180mm => 72m-67f => 67m-67m => 67f-58m => 50mm 180mm => 72m-67f => 67m-67m => 67f-77m => 10-22mm 180mm => 72m-52f => 52m-M25x0.75 => Nikon 10x 180mm => 72m-52f => 52m-M26x36tpi => Mitutoyo 10x 180mm => 72m-52f => 52m-43f => Raynox 250 Magnifications: T2i - 50mm reversed 1x? T2i - 10-22mm reversed lots? T2i - 100mm 1x T2i - 180mm 1x T2i - 100mm - 50mm reversed 2x T2i - 180mm - 50mm reversed 3.6x T2i - 100mm - Nikon 10x 5x T2i - 180mm - Nikon 10x 9x T2i - 100mm - 10-22mm reversed 10x(?)
DOF & #shots: Per this post you need a little overlap, say 20-30%, between the in-focus slabs of adjacent frames Determining number of shots for a stack? DOF Calculator, another Hardware: Nice looking breadboard Nice looking rig w/ details Raynox rig Bratcam - ultimate in positioning Benchmark: Dimensions: Configurations Resolution Contrast Tools: Remote: EOS Utility, Zerene Remote, MikesStepperApp w/ Canon SDK on Mac, w/libgphoto2 & gphoto2 on Linux Camera's focus control, StackShot Zerene Stacker [1] For the Nikon CFI Plan Achromat 10x NA 0.25 WD 10.5mm (p/n MRL00102) reviewed here and here [2] For the Mitutoyo 10x NA 0.28 WD 33.5mm used here and here - WD of 33.5 mm [1] vs [2]
Nikon 10x vs Mitutoyo 10x (discussed here) ![]() |
Nikon 5x vs Mitutoyo 5x (discussed here) ![]() |
Cleaning:
Acetone for 3 days
Stepsize:
DOF Estimates For Macro/Micro
on DOF and Step Size
Overview of Microscope objectives DoF
StackShot:
Fine focusing a microscope using StackShot
-> Tiny focus steps: how to make them, how to measure them
Irregular Steps - StackShot
What does StackShot "High Precision" mean?


Stackshot and 1 micron steps: - bottom of initial post
[Zerene] StackShot Controller Interface
Is it better to use a focus rail or the ring on my lens?
Post Processing:
Suppress DMap artifacts with editing cheat [Zerene]
Camera:
Canon “Live-View” with an electronic first shutter curtain
Wireless to Camera:
Wirelessly tethering Nikon D4 using a sub 50 Euro mini WLAN router
Portable Battery Powered 3G/4G Wireless N Router: TL-MR3040
Reusing StackShot:
StackShot controller incorporated
Slabbing:
Sub-stacks
SlabberJockey slabbing (sub-stacking) utility
Suppress DMap artifacts with editing cheat
EOS Control:
canon-eos-cameras-principles-of-interfacing-and-library-description
ptpusb-control-camera-data
digital-camera-control-using-arduino-usb-host-shield-part-1-basics
3D:
3D scan of fly head
MeshLab
Acetone for 3 days
Stepsize:
DOF Estimates For Macro/Micro
on DOF and Step Size
Mitutoyo is specifying single-sided DOF = maximum deviation from the plane of sharpest focus, as opposed to the two-sided DOF that we want for focus stacking and is computed by Nikon
...
So what step size should you use? Well, it depends. If you plan to stack using PMax, then you can get a sharper rendering "out of the box" by using a smaller step and letting PMax do some sharpening for you as a side effect. Or if stack depth is important, you can take Nikon's smallest number and cut it back by 20% or so to be sure of not missing anything.
A new way of thinking and calculating about DOF...
So what step size should you use? Well, it depends. If you plan to stack using PMax, then you can get a sharper rendering "out of the box" by using a smaller step and letting PMax do some sharpening for you as a side effect. Or if stack depth is important, you can take Nikon's smallest number and cut it back by 20% or so to be sure of not missing anything.
Overview of Microscope objectives DoF
StackShot:
Fine focusing a microscope using StackShot
-> Tiny focus steps: how to make them, how to measure them
Irregular Steps - StackShot
What does StackShot "High Precision" mean?


Stackshot and 1 micron steps: - bottom of initial post
When scrolling through images there is consistent evidence of nutation [nodding] from frame to frame. It's very consistent: one frame slightly to the left and the other back again. So consistent that away from the centre and perpendicular to the direction of nutation the trails from "stuck pixels” in the stack form two parallel tracks like this:
Tiny focus steps: how to make them, how to measure them[Zerene] StackShot Controller Interface
Is it better to use a focus rail or the ring on my lens?
Post Processing:
Suppress DMap artifacts with editing cheat [Zerene]
Camera:
Canon “Live-View” with an electronic first shutter curtain
Wireless to Camera:
Wirelessly tethering Nikon D4 using a sub 50 Euro mini WLAN router
Portable Battery Powered 3G/4G Wireless N Router: TL-MR3040
Reusing StackShot:
StackShot controller incorporated
Slabbing:
Sub-stacks
SlabberJockey slabbing (sub-stacking) utility
Suppress DMap artifacts with editing cheat
EOS Control:
canon-eos-cameras-principles-of-interfacing-and-library-description
ptpusb-control-camera-data
digital-camera-control-using-arduino-usb-host-shield-part-1-basics
3D:
3D scan of fly head
MeshLab