My Micro Photography Gallery

Butterfly Wings
Armenia, 12 Apr 2019
Here is a closeup of the wing of a Sunset Moth, taken using my Mitutoyo 10X objective. 
 
I could see making prints of mosaics or collages of say, these scales from half a dozen or a dozen different subjects.  Printed large in a grid on canvas.  Or say, eyes (human, mammal, insect).

Crystals
Armenia, 6 Apr 2019
Crystals can be grown on a microscope slide and then photographed using polarizing filters.  There is an effect called Birefringence that results in amazing patterns in some materials.
 
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Lake Placid, 2 Jun - 4 Jul, 2019
Below are some images I have made.  They are from microscope slides prepared by yodalovedme (eBay).  I will prepare my own slides in future.
 
Most of these images were taken at 20X magnification and are approx. 2 mm across.  The rest were at 10X are approx. 4 mm across.  Please click on an image to enlarge it (then the browser's back button to return) or click on the Size options to the left to resize all the images.  The first one on the left is presently my favorite.
Hippuric Acid
0021
0022
0023
0024
0039
0046
0041
0042
0047
0048
0040
0043
0044
0045
Ascorbic acid (Vitamin C)
0001
0002
0003
0004
0069
0070
0071
0072
Saccharine
0005
0006
0007
0008
GABA (gamma-Aminobutyric acid, an amino acid)
0009
0010
0011
0012
DHEA (Dehydroepiandrosterone)
0013
0014
0015
0016
Potassium Chlorate
0017
0018
0019
0020
Aspirin
0025
0026
0027
0028
Pregnenolone
0029
0030
0031
0032
Caffeine
0033
0034
0035
0036
Resorcinol
0037
0038
Maleic and Salicylic Acid
0049
0050
0051
Adipic Acid
0053
0054
0055
0056
Niacin
0057
0058
0059
0060
Thioacetamide
0061
0062
Ethylparaben
0063
0064
0065
0066
0067
0068
Below is a sampling from Robert Berdan, Loes Modderman and others.  None of the following photos are mine.
paracetamol/menthol
DMSO with Thiocarbamide, Pol+Ret, 10x objective, Olympus BH2
a mixture of Taurine and Glutamine, pol +ret, 10x objective, water solution
several acids
Ephedrine / imiprazimi
mainly Urea and Glutamine. Pol+ret, alcohol and water solution
Callus remover and Menthol, pol+ret , Alcohol solution
noxacorn/ paracetamol
noxacorn/paracetamol
noxacorn/paracetamol
several acids, pol+ret, water solution
Urea and Salicylic acid
Resorcinol, pol+ret, alcohol solution,10x
noxacorn/paracetamol
Hydroquinone and Glycin, pol + ret, alcohol and water solution, heated
lactic acid and acetylsalicylic acid, alcohol, 10x objective, Pol
Copper sulphate, pol+ret, water solution, 10x objective
decalcifier, water solution, pol+ret
Urea and Glycin, water solution, pol+ret
callus remover recrystallized several times in aceton
Ureum, Salicylic acid and Lactic acid, water and alcohol, 4x objective, pol+ret
maleinic acid and a sweetener, in water solution
A sweetener, water solution, 10x objective, pol+ret
"Maleic acid and dextrine, pol+ret, 10x objective, Olympus BH-2 and Olympus OM D-E-M5 markII camera body with photo objective 10x in the camera adapter tube. So the magnification is at least 100x."
urea and tartaric acid, pol, no retarder, 10x objective, water and alcohol solution
Trimethyl and TRIS, water solution
Menthol, melted, with addition to counteract evaporation.Pol + ret, 10x objective
Caustic Soda and industrial salt, water solution, pol+ret
ditto
ditto
fertiliser and Tartaric acid, 20x objective, pol
guanidine
"Experimented with several kinds of artificial sweeteners today, which gave unexpected results, and disappointments too. Sometimes the producer does something to his product without consideration for crystalmakers. But this one was fine, nice patterns. Some tablets in water."
malonzuur
Maleic acid and TRIS, pol+ret , water and alcohol solution, 10x
 (Lots) More ...
2-4 dichloro A
Trimethyl and TRIS, water solution
Paracetamol, Alcohol, pol+ret
Experiment with Rheinberg illumination. Saccharine and Stevia, water solution
Hydroquinone and a sweetener, alcohol + water, pol+ret, 4x
Magnesium sulfate, from water solution, pol+ret
imiprazimi
atelolum
Thiocarbamide and a fertilizer, DF, converted to monochrome. Water solution, 10x objective
ditto
ditto
Epsom salt, pol+ret, water solution, 10x objective
Paracetamol and Saccharine in water solution, pol+ret
some amino acids from a fitness supplier
thiocarbamide and trimethyl, water solution, 10x
Callus remover, acetone, 10x
TRIS, water solution, pol+ret
Benzoic acid, Salicylspiritus and Tartaric acid
callus remover in aceton. 10x objective
callus remover in aceton, pol+ret
Urea, Glycine and one other, water solution, 10x objective
Magn sulphate, water solution
Niacinamide (Vit B3) and L-Asparagine, water solution. Pol+ret, 10x objective
Vit C and Na carbonate
Citric Acid
Src
Urea
Src
Urea,coffee?,water
Src
Niacinamide (Vit B3)
Hydroquinone,alcohol
Src
Resorcinol
Src
Ascorbic acid,water
Src
Tartaric acid
Aspirin
Resorcinol, alcohol
Ureum, water
Urea, water
Magnesium sulphate
Paracetamol
Hydroquinone+an addition
Tartaric acid and Saccharine
Urea, Paracetamol
Src
Aspirin and Paracetamol under polarized and oblique lighting
Src
Tartaric acid, Dextrin
Na tetraboraat and Glycine
Paracetamol
Paracetamol
Paracetamol
Paracetamol, betamethason
Paracetamol, betamethason
Aqueous resorcinol
Src
Betamethason, paracetamol
Src
Resorcinol
Urea + an acid
Paracetamol + addition, alcohol
Urea + addition, water
N/A
Betamethason/paracetamol A
Barnsteenzuur
Copper Sulfate
Epsom salt
Src
Epsom salt
Epsom salt
Urea
Copper sulphate, in gel
Ammonium di hydrogenfosphate
NAS
Tartaric acid
Salicillic acid and Propylhydroxybenzoate from alcohol solution
Urea and citric acid
Urea + citric acid, water solution
Na thiosulfate, water solution
Urea
Glycine
Progesteron
Tartaric acid after some time
Propylhydroxybenzoate and paracetamol
Thiocarbamide and three others, water solution
Malic acid and softener
Boric acid, mainly
Hydroquinone
Paracetamol, Alcohol solution
Progesteron
Resorcinol M
Hydroquinone and 2 other components
Resorcinol A
Propylhydroxybenzoate
Imidiazole
Malic Acid and Ibuprofen
Atropine
Penylephrini hydr
Efedzine / Hydroquinone
Tartaric acid and menthol
Hydroquinone and Urea
Resorcinol
Paracetamol
Paracetamol and wodka
Boric acid, Sacchanrie and at least 2 others, from water solution
4-hydrobenzoic B 2
TRIS and Glaubersalt, mainly
Oxalic acid
Tartaric acid, in water
kaliumwaterstofphatalaat A
kaliumwaterstofphatalaat
TRIS
Thiocarbamide and Rochellesalt
Mainly Acetylsalicylic acid
Resorcinol and Oxalic acid
Thiocarbamide and an industrial salt
Epsom
Recorcinol, alcohol solution
Resorcinol
"Snus is a traditional form of smokeless tobacco in Sweden. Images are an airdried water extract of a typical bergamot-flavored snus (pouch soaked in water for a few minutes). I am guessing the crystals are mainly salt. Polarized light (no retarder). Image is a stitch of 16 images. 4X objective."
"BIG crystal growth (if that's what it is on 1mm²) from Hydroquinone and a soft drink. Fascinating because they never touch other groups of similar crystals, but intrusions of a different kind are added without a problem. For people who want to learn something about crystallization !"
"The proportion of the two solutes is critical to achieving these forms - I generally start from a solution of 4:1 (v/v) of beta-alanine to l-glutamine and vary concentrations until I get something that works. Ensure that your slides are extremely clean - if they are even slightly hydrophobic the results are generally unusable."
Urea and an addition, water solution, 150x
Thiocarbamide and Rochellesalt
Hydroquinone, alcohol solution
p-hydroxyphenethyl alcohol
4-hydrobenzoic acid G
8-CHINLINOLSULFAAT
8-CHINLINOLSULFAAT
Omeprazole
3/4 hyldroxy phenyl G
Urea, Gluconic acid and an addition
Ijzermagnesiumnitraat
Resorcinol and vitamin drink
Boric acid, Sacchanrie and at least 2 others, water solution
From a garden fertilizer
Diclofenac, water solution
TRIS in water solution
Oxalic acid with resorcinol
TRIS, water solution
Resorcinol. water solution
Pyridoxin and Betaine
3/4 hyldroxy phenyl
Resorcinol, alcohol solution
Oxalic acid and an energy drink
Coffeine and Saccharine, water solution
Urea and gluconic acid
Saccharine and Dextrin, in water solution
Succinic acid and Gluconic acid, water solution
Testosteron Propinate C
Acetamide
Urea and others, water solution
Tartaric acid
From several acids
Urea
Gallic acid and Hippuric acid, water solution
A callus remover
Saccharine and Dextrin, in water solution
Resorcinol, thiocarbamide and a drink, water solution
Hydrochinon in stripes, after a drop of drink, alcohol solution
4-aminobenzoëacid
Nicotinezuur A
Na hydroxide in a special mixture, water solution
Guanidine HCL
n/a
Citric and Ascorbic Acid
Citric and Ascorbic Acid
Thiocarbamide and another component
A sheet of gelatin, stereo, 20x, multicolor filter
DL Tropiczuur A
From several acids
"Extract of white snus. White snus is based on the traditional Swedish oral tobacco product but instead of containing tobacco, it contains a gum base to which nicotine and flavours have been added (mint flavour in this case). I took one sachet of snus and allowed it to steep in a 50:50 mix of water and alcohol for 10 minutes I then filtered the liquid through kitchen towel before leaving to airdry on a slide for a week. Images are taken using x4 objective and simple polarized light (no retarder). Slide shown as well for reference."
Malic Acid + sugar
Tartaric acid, with and without retarder
3/4 hyldroxy phenyl
Benzotriazole
3/4 hyldroxy phenyl
Urea and Boric acid
Nicotinamide
Paracetamol and Salicylic acid
A "special concoction", water solution
Hydroquinon and other components, Alcohol
Resorcinol
Hydroquinone with an addition to straighten them out, alcohol and water
Hydroquinone, mostly
Saccharine, water solution
Nicotinamide
Nicotinamide A
Tartaric acid
Fertilizer, water solution
Malic Acid + sugar
Malic acid, from water solution
KA-Chloraat
"Copper sulphate, taken 5 seconds after each other. First the small crystals appear, and then a wave takes over in a second. In the meantime the crystals keep growing, as you can see in the right corner. This is typical for this chemical."
Magnesiumchloride
4-hydrobenzoic acid
Sulpher
Sulfur
Hydrochinon and glycine, alcohol solution
Progesteron, water solution
magnesium sulphate and several others
Sweeteners and Glycine
Magn sulphate, water solution
Paracetamol/vanille B
Vanilla b
Resorcinol AK
Hydroxy B
Hydroquinone and Glycine
Vanille/paracetamol D
Vitamine C/Salicylzuur
Vanille/paracetamol
Magnesiumchloride
Creatine and Na thiosulphate
Hydroquinone D
Benzotriazole
Magnesium sulphate, water solution
Antron
Bemegride
Ammonium Sulphate
Gluconic acid, water solution
p-hydroxyphenethyl A
Boric acid and another acid
Crystals that would need stacking. Combination of three colorful chemicals in water solution, 150x
Hydroquinone and Thiocarbamide
Magnesium sulphate, water solution
Hydroquinone and Thiocarbamide
Resorcinol, alcohol, 150x
Na-methoxyde
Na-methoxyde
Na-methoxyde F
Na-methoxyde C
Urea and Boric acid, water solution, 150x pol+ret
Amm thiosulphate and Na thiosulphate, water solution
Resorcinol and Tartaric acid, alcohol solution, 150x
Boric acid and Thiocarbamide
Callus remover liquid
A mixture of sugars, water solution
Boric acid and Urea, millions of little crystals popping up. Water solution
Urea and Boric acid, water solution. Less them 1mm²
Urea and some acids
Thiocarbamide and Ascorbic acid, water solution, pol+ ret , 150x
Na-methoxyde C
D-threo-2-amino
Pyrocatechine
Boric acid and Thiocarbamide, water solution, 150x
Resorcinol and Tartaric acid, alcohol solution, 150x
D-threo-2-amino.B
Gluconic acid
methoxyphenyl
Several kinds of fertilizer, water solution
D-threo.amino-1 A
Several acids
Malonic
Hydroquinone, Glycine and wodka
Oxalic acid pattern, alcohol
One of 20 years ago, from Urea , water solution
Resorcinol, alcohol solution, pol+ret, 100x
References:
   - How to Photograph the Magical Microscopic World – Photomicroscopy
   - The Art & Science of Photomicrography with Polarized Light, Dr. Robert Berdan, June 22, 2017
   - Crystals Photographed with Polarization Microscopy: Water, Beer, Caffeine, Vitamins, Amino Acids and Human Tears, Dr. Robert Berdan, March 17, 2019
   - Video: DIY polarized light microscopy of Vitamin C Crystals
   - The Seven Most Common Grades for Chemicals and Reagents:
  1. ACS grade meets or exceeds purity standards set by the American Chemical Society (ACS). This grade is acceptable for food, drug, or medicinal use and can be used for ACS applications or for general procedures that require stringent quality specifications and a purity of ≥95%.
  2. Reagent grade is generally equal to ACS grade (≥95%) and is acceptable for food, drug, or medicinal use and is suitable for use in many laboratory and analytical applications.
  3. USP grade meets or exceeds requirements of the United States Pharmacopeia (USP). This grade is acceptable for food, drug, or medicinal use. It is also used for most laboratory purposes, but the USP being followed should always be reviewed prior to beginning to ensure the grade is appropriate for that methodology.
  4. NF grade meets or exceeds requirements of the National Formulary (NF). The USP and the NF (USP– NF) jointly publish a book of public pharmacopeial standards for chemical and biological drug substances, dosage forms, compounded preparations, excipients, medical devices, and dietary supplements. The listings here should be reviewed to determine which would be considered equivalent grades.
  5. Laboratory grade is the most popular grade for use in educational applications, but its exact levels of impurities are unknown. While excellent for teaching and training, it is not pure enough to be offered for food, drug, or medicinal use of any kind.
  6. Purified grade, also called pure or practical grade, meets no official standard; it is not pure enough to be offered for food, drug, or medicinal use of any kind.
  7. Technical grade is used for commercial and industrial purposes; however, like many others, it is not pure enough to be offered for food, drug, or medicinal use of any kind.
      ACS, Reagent, and USP-NF grades are typically equivalent and interchangeable
FIN